Backround: The aim of this work was to investigate the antimicrobial, antioxidant, and antimutagenic potentials of methanol extracts from
E.
angustifolia.
Materials and Methods: Methanol extracts were screened for antimicrobial activity against different species of 4 Gram positive and 3 Gram
negative bacteria and one fungus. These bacteria included food pathogens. The leaf extract was tested using disc diffusion assay.
Results: The methanol extract of
E. angustifolia showed maximum inhibition zone of 16 mm against
Yersinia enterocolitica
. Whereas, the
inhibition zone was not determined by methanol extract against
Escherichia coli
ATCC 1122 and
Candida albicans
RSKK 02029. The MIC was
evaluated on plant extracts as antimicrobial activity. All of bacterial strains showed the lowest sensitivity to methanol extract of
E. angustifolia
(3.5 mg/mL), except
Yersinia enterocolitica NCTC 11174. In addition, the plant extracts were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical. Finally, the methanol extract displayed a strong antioxidant activity (Trolox equivalent = 1.49 mM). Also,
E. angustifolia methanol extracts were screened for their antimutagenic activity against sodium azide by Ames test in absence of rat microsomal
liver enzyme (-S9). The results showed that
E. angustifolia methanol extracts can inhibit mutagenic agents of sodium azide. The plant leaf
extracts with the inhibition of 36% sodium azide showed moderate potential in decreasing mutagenic agents in
Salmonella typhimurium
TA100.
Conclusion: E. angustifolia methanol extracts have antimicrobial, antioxidant and antimutagenic potential.
