CHINESE HERBAL MEDICINE SHENQI DETOXIFICATION GRANULE INHIBITS FIBROSIS IN ADENINE INDUCED CHRONIC RENAL FAILURE RATS|
Peng, Min; Cai, Pingping; Ma, Hongbo; Meng, Hongyan; Xu, Yuan; Zhang, Xiaoyi & Si, Guomin
Background: Progressive fibrosis accompanies all chronic renal disease, connective tissue growth factor (CTGF,) and platelet-derived growth
factor-B, (PDGF-B,) play important roles in extra-cellular matrix abnormal accumulation, while endothelin -1 (ET-1) nitric oxide (NO,) are
related to endothelial dysfunction, which mediates the progression of renal fibrosis. Shenqi Detoxification Granule (SDG), a traditional Chinese
herbal formula, has been used for treatment of chronic renal failure in clinic for many years.
Materials and Methods: In order to evaluate the efficacy, and explore the mechanism of SDG to inhibit the progression of renal fibrosis, study
was carried out using the adenine-induced Wister rats as the CRF model, and losartan as postive control drug. Levels of serum creatinine [Scr],
and blood urea nitrogen (BUN), albumin (ALB), 24hrs, urine protein (24hUP), triacylglycerol (TG), and cholesterol (CHO), together with ET-1,
and NO were detected. Pathological changes of renal tissues were observed by HE, staining. In addition, CTGF and PDGF-B expression were
analyzed by immuno-histo-chemistry.
Results:The results indicated that SDG can effectively reduce Scr, BUN, 24hUP, TG, and CHO levels, increase ALB levels, inhibit renal tissue
damage in CRF rats, and the mechanism maybe reduce PDGF-B, CTGF expression and ET-1/NO.
Conclusion: Shenqi Detoxification Granule is a beneficial treatment for chronic renal failure.
Chronic Renal Failure; fibrosis; Shenqi Detoxification Granule; CTGF; PDGF-B