In Mexican traditional medicine, the seeds of Byrsonima crassifolia
have been used in the treatment of diabetes, rheumatism and for
wound healing. The aim was to evaluate the effect of seeds of B. crassifolia
on insulin release from the pancreatic beta cells in streptozotocininduced
Materials and Methods:
In the present study, we evaluated the beneficial effect of seeds B. crassifolia
streptozotocin-induced type 1 diabetic rats.
Insulin level; insulin sensitivity index, insulin content in pancreas, malonaldehyde, nitric oxide contents, oxidative stress parameters were assayed.
Serum glucose levels were determined by the glucose oxidase method. To determine the insulin releasing activity, after extract treatment, the pancreas
was excised. Pancreatic sections were processed for examination of insulin-releasing activity using an imunocytochemistry kit.
Administration of the hexane extract (200 and 400 mg/kg), exhibited a significant reduction in serum glucose. Administration of
streptozotocin decreased the number of beta cells with insulin secretory activity in comparison with intact rats; but treatment with the B. crassifolia
seed extract increased significantly the activity of the beta cells in comparison with the diabetic control rats. The extract decreased serum glucose in
streptozotocin-induced diabetic rats and increased insulin release from the beta cells of the pancreas.
These finding suggest that B. crassifolia
seed has beneficial effect for diabetes through decreasing blood glucose and lipid levels,
increasing insulin sensitivity index and insulin content, up-regulating antioxidant enzyme activity and decreasing lipid peroxidation.