Background: Alkaloids of
Sophora alopecuroides
have good biological activity, and are widely used in clinical settings, which not only have
pharmacological activities of anti-cancer, cancer suppression, as well as the inhibition, and killing of various microorganisms; but also possess
extensive pharmacological effects on immune system, nervous system and cardiovascular system. The objective of this paper was to extract and
isolate total alkaloids of
Sophora alopecuroides (TASA), and to study their anti-tumor effects in H22 tumor-bearing mice.
Materials and Methods: TASA were extracted and isolated using thin-layer chromatography, and column chromatography; and the isolated
compounds were analyzed using nuclear magnetic resonance. The inhibitory effects of TASA on tumor in H22-bearing mice were determined by
MTT assay.
Results: Three compounds were isolated from
Sophora alopecuroides L., which were matrine, oxymatrine and sophoridine, respectively. Meanwhile,
mouse H22 sarcoma model was established and different doses of TASA apparently inhibited solid H22-tumor in mice; it inhibited the thymus, and
spleen to some extent; the degree of inhibition was more obvious for the spleen.
Conclusion: TASA has an anti-tumor effect in H22 tumor-bearing mice.
