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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 11, No. 2, 2014, pp. 315-318
Bioline Code: tc14049
Full paper language: English
Document type: Short Communication
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 11, No. 2, 2014, pp. 315-318

 en IN-VITRO SCREENING OF MALAYSIAN HONEY FROM DIFFERENT FLORAL SOURCES FOR ANTIBACTERIAL ACTIVITY ON HUMAN PATHOGENIC BACTERIA
Ng, Wen-Jie; Ken, Khai-Wei; Kumar, Roshani-Vijaya; Gunasagaran, Hemamalani; Chandramogan, Vanaysha & Lee, Ying-Yee

Abstract

Background: Different researches on therapeutic effects of honey have been conducted in different regions; however the study on the potential antibacterial activity of Malaysian honey is still limited. In this study, antibacterial activities of different monofloral honey samples were tested against several common human pathogenic bacteria.
Materials and Methods: The well-diffusion method, minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) techniques were employed to investigate the putative antibacterial activity of Malaysian monofloral honey from Koompassia excelsa check for this species in other resources (Becc.) Taub (Tualang), Melaleuca cajuputi check for this species in other resources Powell (Gelam) and Durio zibethinus check for this species in other resources Murr. (Durian). Honey samples were tested against Staphylococcus aureus check for this species in other resources ATCC6518 and ATCC25923, Staphylococcus epidermidis check for this species in other resources ATCC12228, Enterococcus faecium check for this species in other resources LMG16192, Enterococcus faecalis check for this species in other resources LMG16216 and ATCC29212, Escherichia coli check for this species in other resources ATCC25922, Salmonella enterica check for this species in other resources serovar Typhimurium ATCC14028 and Klebsiella pneumoniae check for this species in other resources ATCC13883.
Results: Marked variations were observed in the antibacterial activity of these honey samples. Durian honey failed to produce substantial antibacterial activity, whereas Tualang and Gelam honey showed a spectrum of antibacterial activity with their growth inhibitory effects against all of the tested bacterial species including vancomycin-resistant enterococci (VRE).
Conclusion: Present findings suggested Gelam honey possesses highest antibacterial effect among the tested Malaysian honey samples.

Keywords
Honey; monofloral; antibacterial; well-diffusion method; VRE

 
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