STUDY ON THE EXTRACTION PROCESS OF TOTALANTHRAQUINONES IN RADIX ET RHIZOMA RHEI AND THEIR ANTILIPEMIC EFFECTS11
Xie, Hui-chun & Shang, Jun
Background: Radix et Rhizoma Rhei has a gastric mucosal protective effect, major anti-gastritis and anti-peptic ulcer active constituents were
emodin, aloe-emodin, chrysophanol, rhein, etc. The objective of the study was the extraction process of total anthraquinones in Radix et Rhizoma
rhei and their antilipemic effects.
Materials and Methods: Orthogonal experiment, UV spectrophotometry and conventional antilipemic effect determination method were used to
optimize the extraction process, and to determine the total anthraquinone content, as well as blood levels of total cholesterol, triglycerides, HDL
Results: Ethanol concentration, extraction time and ethanol amount had significant influences on the extraction of total Radix et Rhizoma rhei
anthraquinones, total Radix et Rhizoma rhei anthraquinones could significantly reduce blood levels of total cholesterol, triglycerides, HDL and
Conclusion: The optimum extraction process was two times extraction of Radix et Rhizoma rhei with 70% ethanol, the amounts of solvent of 8
folds and 5 folds, successively, and the extraction time of 60 min each. In addition, this extract has an antilipemic effect in mice.
Radix et Rhizoma Rhei; total anthraquinones; orthogonal test; anti-lipids