Garlic oil which is the main active constituent of garlic has a wide range of pharmacological activities, and a broad antibacterial
spectrum. It also has a strong anti-cancer activity, and can significantly inhibit a variety of tumors such as liver cancer, gastric cancer and colon
cancer. The objective is to study the extraction process of garlic oil and its antibacterial effects.
Materials and Methods:
Supercritical extraction was used to investigate the optimal processing conditions for garlic oil extraction; filter
paper test and suspension dilution test were applied to determine the bacteriostatic action of garlic oil.
In the CO2
supercritical extraction experiment, factors influencing the yield of garlic oil were: extraction pressure > extraction
temperature > extraction time in descending order. Range analysis showed that the optimal experimental conditions for CO2
extraction of garlic oil were extraction pressure of 15 Mpa, temperature of 40 ℃, and duration of 1 h. Different concentrations of garlic oil could
all inhibit the growth of Staphylococcus aureus
, Escherichia coli
and Bacillus subtilis
, suggesting that garlic oil has an antibacterial effect.
The optimal experimental conditions for CO2
supercritical extraction of garlic oil were: extraction pressure of 15 Mpa, temperature
of 40 ℃, and duration of 1 h; garlic oil has an antibacterial effect.