EVALUATION OF THE ANTINOCICEPTIVE EFFECT OF THE ETHANOLIC EXTRACT OF PUNICA GRANATUM |
Saad, Lamees Ben; Hwi, Kim Kah & Quah, Tony
Background: There are severe adverse effects of analgesic drugs on human body. Extraction of analgesic drugs from natural products has
therefore become the prime objective of the study. In this study, we aimed to evaluate the antinociceptive activity of the pomegranate fruit.
Materials and Methods: Antinociceptive activity of ethanol pomegranate extract was examined using three models of pain: the writhing test, the
hot tail flick test and the plantar test. The ethanolic extract of pomegranate was administered by oral gavages in doses of (100,150 and 200mg/kg,
p.o (orally)), for all the tests and compared with aspirin (100mg/kg, p.o.) which was considered as the standard drug. Phytochemical screening
and HPLC analysis of the plant species was carried out.
Results: In the writhing test, the index of pain inhibition (IPI) was 37% for ethanolic extract of pomegranate (200mg/kg, p.o.), and 59% for
aspirin. In the hot tail flick test, the ethanolic extract of pomegranate (200mg/kg, p.o.), has shown significant analgesia reaching its peak at 60 min
maximum possible analgesia (MPA), was 24.1% as compared with aspirin 37.5%. Hyperalgesia was successfully induced by the plantar test and
the ethanol extract of pomegranate (100,150,200mg/kg, p.o.), reduced the hyperalgesia in a dose dependent manner comparable to aspirin at
(100mg/kg, p.o.). HPLC analysis revealed the presence of gallic acid, ellagic acid and Punicalagins A&B.
Conclusion: The results demonstrated that ethanol pomegranate extract has an antinociceptive effect that may be related to the presence of
Pain; ethanolic extract of pomegranate; analgesia; phytochemicals