Flowers of Bauhinia kockiana
were investigated for their anticancer properties.
Gallic acid (1
), and methyl gallate (2
), were isolated via
bioassay-directed isolation, and they exhibited anticancer properties towards
several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3
) was examined against the same cancer cell lines to deduce the
bioactive functional group of the phenolic compounds.
The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI50
30 - 86 μM),
but were inactive towards DU145 prostate cancer cell (GI50
> 100 μM).
It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer
activity. Also, the carboxyl group of compound 1
was also important in anticancer activity. Examination of the PC-3 cells treated with compound 1
using fluorescence microscopy showed that PC-3 cells were killed by