search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 11, No. 4, 2014, pp. 62-65
Bioline Code: tc14123
Full paper language: English
Document type: Short Communication
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 11, No. 4, 2014, pp. 62-65

 en CYTOTOXICITY OF SELECTED MEDICINAL PLANTS USED IN MT. FRERE DISTRICT, SOUTH AFRICA
Mnengi, Dorris; Kappo, Abidemi; Kambizi, Learnmore & Nakin, Motebang

Abstract

Background: In South African traditional medicine, some are plants known to combat pediatric diseases and are commonly used by traditional healers. The aim was to evaluate cytotoxicity effects of plants.
Materials and Methods: The ground plant material was exhaustively extracted using methanol, acetone and water separately for 72 hrs. These organic solvents were removed from filtrates using a rotavapour. Stock solutions were prepared at 40 mg/ml Dimethyl sulfoxide (DMSO) and test solutions were transferred into vials and 10 brine shrimps introduced in each. The number of dead shrimps was counted to ascertain toxicity. Ten A. salina check for this species in other resources nauplii (larva) were transferred into each sample vial and filtered brine solution was added to make 5 ml. The nauplii were counted macroscopically in the stem of the pipette against a lighted background. A drop of dry yeast suspension was added as food to each vial. Probit analysis was used to determine the concentration at which lethality to brine shrimp represents 50 % (LC50).
Results: All the tested extracts showed that the concentration is directly proportional to death of brine shrimps. Fifty percent lethality (LC50) of the tested crude extract ranged between 4.1 and 4.6 μg/ml with methanol extract of B. abyssinica check for this species in other resources being the lowest and T. acutiloba check for this species in other resources the highest.
Conclusion: This study revealed that 100% of plant crude extracts screened for activity against Artemisia salina larvae showed strong cytotoxicity below 10 μg/ml and plant species with LC50 values < 1000 μg/ml may not make good paediatric remedies due to their inherent toxicity.

Keywords
Medicinal plants; traditional pediatrics; cytotoxicity

 
© Copyright 2014 - African Journal of Traditional, Complementary and Alternative Medicines
Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil