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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 11, No. 4, 2014, pp. 136-141
Bioline Code: tc14134
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 11, No. 4, 2014, pp. 136-141

 en COMBINATIVE EFFECTS OF THANH HAO MIET GIAP THANG (SWEETWORMWOOD AND TORTOISE SHELL DECOCTION) INGREDIENTS ON ANTIOXIDATIVE ACTIVITY IN VITRO
Nuong, Nguyen My; Vy, Nguyen Thuy & Duong, Ho-Huynh Thuy

Abstract

Background:Traditional formulae usually exhibit therapeutic effects through the combinations of different ingredients. The purpose of this study was to investigate in vitro anti-oxidative activity of Thanh Hao Miet Giap Thang (THMGT) (Sweet Wormwood and Tortoise Shell Decoction) formula and the interactions of its ingredients leading to the overall anti-oxidative effect.
Materials and Methods: We prepared 31 combinations containing two to four of the five ingredients including Herba Artemisia apiacea check for this species in other resources L (HbA), Carapax Trionycis ( Tryonix sinensis check for this species in other resources ) (CT), Rhizoma Anemarrhenae ( Anemarrhena asphodeloides check for this species in other resources ) (RzA), Radix Rehmanniae ( Rehmannia glutinosa check for this species in other resources Libosch) (RdR), Moutan Cortex ( Paeonia suffruticosa check for this species in other resources ) (MC). These combinations were tested for anti-oxidative activity using DCFH-DA and DPPH assays on Hep G2 cells. We also analyzed changes in expression of genes involved in antioxidant defense system including Nuclear Factor Erythroid- Derived 2-Like 2 (NFE2L2), catalase (CAT), heme oxygenase-1 (HO-1), glutathione peroxidase (GPx), cytoplasmic superoxide dismutase (SOD1), mitochondrial superoxide dismutase (SOD2).
Results: The complete formula and all combinations containing Moutan Cortex showed high antioxidant activity in both radical solution-based chemical assay and cellular-based assay. On the contrary, Carapax Trionycis displayed inhibitory effect on the overall antioxidant activity when present in a combination, an effect clearly emphasized in cellular-based assay. Hep G2 cells treated with the formula showed increased gene expression of HO-1 and SOD2 while expression of CAT, SOD1, GPx was unchanged.
Conclusion: Our results suggested that THMGT had anti-oxidative activity essentially through intrinsic reducing capacities and the overall activity of the formula resulted from enhancing and inhibiting interactions of ingredients.

Keywords
Thanh Hao Miet Giap Thang; Sweet Wormwood and Tortoise Shell Decoction; antioxidant; traditional formula

 
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