INHIBITION OF THREE NOVEL RADIX SCUTELLARIAE EXTRACTS ON IMMEDIATE HYPERSENSITIVITY|
Li, Xiaorong; Wang, Jiangning; Shi, Haiyun; Gao, Lei & Wang, Xueyan
Background: Radix Scutellariae, a few papers reported its pharmacology activities including alleviate small intestines smooth muscles spasm,
sedation, antihypertensive effect. However, the inhibition of its different organic extracts on immediate hypersensitivity has not bee researched.
Materials and Methods: To investigate the anti-immediate hypersensitivity of three extracts including ethanol extracts, acetone extracts, ethyl
acetate extracts from Radix Scutellariae, four pharmacological screening model were chose, such as 4-Aminopyridine induced pruritus model,
histamine-induced mouse paw edema model, PCA (passive cutaneous anaphylaxis) in ear of mouse, activie cutaneous anaphylaxismouse (mouse
ear edema test), furthermore, total IgE level in the sensitized mice serum was evaluated deeply.
Results: Ethanol group at 1.42g/kg and 0.71g/kg could greatly decrease the licking number to 1.2 and 12.7 respectively; also keep mice paw
swelling at 0.29ml and 0.51ml at 15 min after injection of histamine. Both ear passive cutaneous allergic reaction and active cutaneous
anaphylaxis-ear swelling test demonstrated that ethanol group exhibit great inhibition on immediate hypersensitivity. Low IgE level was found in
ethanol group, but high in other two groups.
Conclusion: The ethanol extracts exhibits obvious strong inhibition, however, the acetone ones and ethyl acetate showed a little.
Radix Scutellariae; Ethanol extracts; Anti-allergic activity; PCA; IgE level