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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 11, No. 5, 2014, pp. 99-104
Bioline Code: tc14153
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 11, No. 5, 2014, pp. 99-104

 en ANTI-TUMOR EFFECT OF CACTUS POLYSACCHARIDES ON LUNG SQUAMOUS CARCINOMA CELLS (SK-MES-1)
Li, W.; Wu, D.; Wei, B.; Wang, S.; Sun, H. X.; Li, X. L.; Zhang, F.; Zhang, C. L. & Xin, Y.

Abstract

Background: Cactus polysaccharides are the active components of Opuntia dillenii check for this species in other resources which have been used extensively in folk medicine. In this study, we investigate the anti-tumor effect of cactus polysaccharides on lung squamous carcinoma cells SK-MES-1.
Materials and Methods: The inhibitory effect of Cactus polysaccharides on lung squamous carcinoma cells were detected by MTT assay. Cell cycle was determined by flow cytometry and cell apoptosis was determined by AnnexinV assay. Western-blotting was applied to detect P53 and PTEN protein expression in the cells treated with cactus polysaccharides.
Results: Results showed that different concentrations of wild cactus polysaccharides prevent SK-MES-1 cells growth and induces S phase arrest. The data also revealed that cactus polysaccharides cause apoptosis in SK-MES-1 cells determined by Annexin-V assay. Furthermore, cactus polysaccharides induced growth arrest and apoptosis may be due to the increase of P53 and phosphatase and tension homolog deleted on chromosome ten (PTEN) protein.
Conclusion: Cactus polysaccharides have anti-tumor activity on lung squamous carcinoma cells.

Keywords
Cactus polysaccharides; Lung squamous carcinoma; Anti-tumor effect; P53; PTEN

 
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