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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 12, No. 1, 2015, pp. 1-8
Bioline Code: tc15001
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 1, 2015, pp. 1-8

 en POLYPHENOLIC CONTENT AND IN VITRO ANTIOXIDANT EVALUATION OF THE STEM BARK EXTRACT OF PELTOPHORUM AFRICANUM check for this species in other resources SOND (FABACEAE)
Okeleye, B.I.; Nongogo, Vuyokazi; Mkwetshana, Noxolo T. & Ndip, R.N.

Abstract

Background: Peltophorum africanum check for this species in other resources has been traditionally used to relief stress induced diseases. The study was aimed to evaluate the antioxidant activities of ethyl acetate extract.
Material and Methods: The in vitro antioxidant activities of Peltophorum africanum stem bark extract was examined in this study by means of +radical scavenging and ferric reducing power analysis using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2`-azino-bis (3-ethylbenzthiazoline-6- sulfonic acid (ABTS) kit, hydrogen peroxide (H2O2), iron (iii) chloride (Fe3+) and nitric oxide (NO). In assessing the likely effects of secondary metabolites on the activities observed; total proanthocyanidins, phenolics, flavonols, and flavonoids were determined using standard phytochemical methods. Data was analyzed by ANOVA test and the p-value < 0.05 was considered significant.
Results: Extract scavenging activity of 88.73± 6.69 % (25 μg mL-1), 53.93±1.09 % (25 μg mL-1), 87.293±6.64 % (25 μg mL-1), 10.55±2.16 mM (0.42 mM) and 3.8115±0.06 (25 μg mL-1) were recorded for H2O2, NO, DPPH, ABTS and Fe3+ reducing power respectively. These values were comparable to the standard compounds; DBPC*BHT, L (+) - Ascorbic acid and Trolox™ (p < 0.05). Proanthocyanidins (92.18±4.68 mg/g) occurred more (p < 0.05) in the extract when compared to all other compounds tested: phenolics (60.53±1.46 mg/g) > flavonoids (18.37± 2.11 mg/g), > flavonols (11.20±3.90 mg/g). However the difference between flavonols and flavonoids was not significant (p > 0.05) at 95% confidence interval.
Conclusion: The results of this study validated the folkloric use of P. africanum which could be exploited as an easily available and a cheaper source of natural antioxidants.

Keywords
Peltophorum africanum; antioxidant; phytochemicals; polyphenolics; radicals

 
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