Plants have always been a successful source of remedy from nature. With the widespread use of medicinal plants by indigenous people,
the search for biologically active agents is relevant as these plants have the potential to provide pharmacologically active compounds. This study
aimed for investigating the effect of antioxidant and antibacterial properties of Ziziphus mucronata
Willd (Rhamnaceae) and Ricinus Communis
aluminum-backed TLC plates (Fluka, silica gel F254), vanillin-sulphuric acid reagent, Fehling’s solution, concentrated sulphuric acid,
ferric chloride, Drangendorff’s reagent, acetic anhydride, 2, 2-diphenyl-1-picrylhydrazyl (DPPH), p
Antibacterial activity was evaluated using microdilution assay and bioautography. Antioxidant activities were determined by using 2, 2-
diphenyl-1-picrylhydrazyl (DPPH). In vitro cytotoxicity was determined using the tetrazolium-based colorimetric assay.
Results: R. communis
leaves had eight secondary metabolites. Quantitative assay for R. communis
, chloroform and methanol extracts had very high
antioxidant activity compared to vitamin C. Plants extracts from all solvents exhibited high antibacterial activity against Staphylococcus aureus
, Escherichia coli
and Pseudomonas aeruginosa
with minimum inhibitory concentration (MIC) values between 0.21 and 1.05
mg/ml. Most of the antibacterial compounds observed on bioautography had Rf
values ranging from 0.21 to 0.88. Z. mucronata
had LC50 of 105.5
μg/ml and R. communis
131.8 μg/ml on Vero cells.
This study revealed that both plants had free radical scavenging and antibacterial activities.