African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 12, No. 1, 2015, pp. 109-114
Bioline Code: tc15016
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 1, 2015, pp. 109-114
© Copyright 2015 - African Journal of Traditional, Complementary and Alternative Medicines
SYNERGISTIC EFFECT OF RADIATION AND TRADITIONAL CHINESE MEDICINE RHIZOMA TYPHONII ETHANOL EXTRACTS DEPENDS ON P53 EXPRESSION IN TREATMENT OF LEWIS MOUSE LUNG CANCER CELLS|
Zhao, Yinlong; Zhao, Lingjun; Luo, Yunxiao; Li, Xin; Zhang, Yifan; Liu, Xiaodong; Luo, Yungang & Zhong, Lili
Background: Lung cancer is the leading cause of cancer-related death, and it is the most common cancer in terms of both incidence and mortality.
There is an urgent need on novel therapeutic strategies for lung cancer. Traditional Chinese herbal medicines (CHM) have potential valuable for
Materials and Methods: Lewis mouse lung cancer cell line and Lewis cells tumors xenograft were used in this experiment. MTT assay was used
to detect cell proliferation, flow cytometry (FCM) analysis and Western blotting to detect cell apoptosis, and colony formation assay to evaluate
the effect of combined therapy of RT+IR.
Results: Our data showed that Rhizoma typhonii (RT) obviously inhibited the proliferations of Lewis cells in time and dose dependent manners
by MTT assay and enhanced radiosensitivity by colony formation assay. The effects of RT to Ionizing radiation (IR) therapy were demonstrated
radiosensitivity on tumors xenograft experiment. In our study, RT induced apoptotic in Lewis cells directly and enhanced the pro-apoptotic effect
of IR by regulating the expression of p53.
Conclusions: These data suggested that RT may be a great potential anti-tumor medicine and the combination of RT and IR may provide a new
therapeutic strategy for the treatment of Lewis lung cancer.
Rhizoma Typhonii; Radiation; Lung cancer; Combined therapy; Apoptosis
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