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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 12, No. 2, 2015, pp. 55-59
Bioline Code: tc15028
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 2, 2015, pp. 55-59

 en ANTI-GIARDIA ACTIVITY OF HEXANE EXTRACT OF CITRUS AURANTIFOLIA check for this species in other resources (CHRISTIM) SWINGLE AND SOME OF ITS CONSTITUENTS
Domínguez-Vigil, Irma Guadalupe; Camacho-Corona, María del Rayo; Heredia-Rojas, J. Antonio; Vargas- Villarreal, Javier; Rodríguez-De la Fuente, Abraham O.; Heredia-Rodríguez, Omar & Mata-Cárdenas, Benito David

Abstract

Background: Giardia lamblia check for this species in other resources is a parasite that causes giardiasis in humans and other mammals. The common treatment includes different drugs, which were described to produce unpleasant side effects. Citrus aurantifolia check for this species in other resources , popularly known as “lima”, is a plant used in traditional medicine to treat gastrointestinal symptoms. The aim of the present study was to evaluate the anti-Giardia activity of 10 pure compounds obtained from a hexanic extract of Mexican lime on the basis of trophozoite growth inhibition.
Materials and Methods: A hexanic extract obtained from fresh fruit peels of Citrus aurantifolia was tested on G. lamblia strain 0989:IMSS trophozoites cultured in TYI-S-33 medium. The concentration of all standard drugs, analyzed by gas chromatography, was adjusted at 10 mg/mL. Metronidazole was used as a positive control. Growth inhibition was determined by counting the number of trophozoites using a Neubauer chamber. The 50% inhibitory concentration (IC50) of each drug was calculated by probit analysis and 95% confidence limits were calculated.
Results: 4-hexen-3-one, citral and geraniol showed IC50 values of 34.2, 64.5 and 229.49 μg/ml in axenic cultures after 24 hr of incubation, respectively. When these results were compared with a positive control of metronidazole; 4-hexen-3-one was 66 times; citral was 112 and geraniol was 441 times less active respectively. The other tested compounds did not inhibit the growth of cultured G. lamblia trophozoites.
Conclusion: The obtained results lead us to propose that these tested compounds from C. aurantifolia have potential for use as therapeutic agents against giardiasis.

Keywords
antigiardial; Citrus aurantifolia; antiprotozoal activity; Giardia lamblia

 
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