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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 12, No. 2, 2015, pp. 126-132
Bioline Code: tc15037
Full paper language: English
Document type: Short Communication
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 2, 2015, pp. 126-132

 en IN VITRO WOUND HEALING ACTIVITY OF SAPIUM INDICUM check for this species in other resources WILLD LEAF EXTRACTS
Azis, Huwaida Abdul; Taher, Muhammad; Susanti, Deny & Zakaria, Zainul Amiruddin

Abstract

Background: Sapium indicum check for this species in other resources Willd (Euphorbiaceae) is traditionally used to accelerate wound healing process in Malay community. Objective: To evaluate wound healing potential of aqueous (AESI) and ethanolic extract of S. indicum leaves (EESI) using cell migration model.
Methods: AESI and EESI were prepared using maceration techniques. The extracts were subjected to phytochemical screening, cytotoxicity (MTT assay) and scratch assay.
Results: AESI contained saponins and tannins while EESI contained triterpenes, tannins and proteins. Based on MTT assay, AESI and EESI exhibited IC50 = 16.3 and 70.9 μg/mL, respectively. In the scratch assay, EESI caused significant (P< 0.05) concentration-dependent migration on 3T3 L1 cells whereas AESI exerted concentration-independent effect.
Conclusion: The leaves of S. indicum exhibited wound healing potential and contained phytochemicals that may contribute to the activity. These findings would support potential use of S. indicum as wound healing plant.

Keywords
Sapium indicum; cell migration; in vitro wound healing

 
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