Background: In traditional medicines, the root of
CNESTIS PALALA
was used for the treatment of stomach ache, malaria, urinary track disorders and
snakebite. The seed was used for poisoning rat and stray dogs. However, the bioactivities and chemical constituents have not been reported. So,
this will be the first report.
Material and Methods: Biological investigations of
C. palala extracts against bacteria (
Staphylococcus aureus
,
Staphylococcus epidermidis
,
Propionibacterium acnes
,
Escherichia coli
, and
Pseudomonas aeruginosa
), fungi (
Trichophyton mentagrophytes
,
Trichophyton rubrum
and
Microsporum gypseum
), yeast (
Candida albicans
), tuberculosis (
Mycobacterium tuberculosis
), malaria (
Plasmodium falciparum) and cancer cell
lines (MCF-7 and HT-29) were evaluated. The chromatographic and spectroscopic techniques were used for the isolation and identification of
pure compounds.
Results: The results showed that the ethanol leaf and bark extracts were active against
S. aureus and
S. epidermidis. The hexane leaf and seed
extracts and petroleum ether bark and root extracts showed strongly inhibition values against MCF-7. Moreover, the petroleum ether bark extract
exhibited high inhibition value against HT-29. Seven compounds were isolated as
β-sitosterol-glucoside (
CP1), hydroquinone (
CP2),
β-sitosterol
(
CP3), mixture of fatty acids (
CP4) and ethyl caffeate (
CP5), scopoletin (
CP6) and 2-nonenal (
CP7). The
CP2 was strongly active against
S.
aureus and
S. epidermidis, the MIC was showed 30.10 μg/ml and 15.05 μg/ml and the MBC was showed 15 μg/ml and 7.5 μg/ml, respectively.
Conclusion: These results suggested that
C. palala is a great potential source of anti-microbial agents. Hence, this is the first study, which will
be the database for chemical constituents and bioactivities of
C. palala.
