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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 12, No. 3, 2015, pp. 85-90
Bioline Code: tc15052
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 3, 2015, pp. 85-90

 en IN VITRO IMMUNE-MODULATORY POTENTIAL OF CRUDE EXTRACT OF LEAF OF ALBIZIA GUMMIFERA check for this species in other resources AGAINST STIMULATED PERIPHERAL BLOOD MONONUCLEAR AND RAW CELLS
Makgatho, M.E.; Mashele, S.A.; Makhubele, T.G.; Sedibane, M.L.; Bagla, V.P.; Mashinya, F.; Mokgotho, M.P.; Masoko, P.; Makola., R. & Matsebatlela, T.M.

Abstract

Background: Albizia check for this species in other resources species including A. GUMMIFERA check for this species in other resources have been used in folk medicine for the treatment of various conditions by many cultural groups. Apart from its anti-parasitic and antimicrobial activity associated with its use, Albizia gummifera have not been investigated for either its anti-inflammatory properties or anti-proliferative effect in vitro.

Materials and Methods: Extracted acetone crude extract of plant material was tested for the presence of various secondary metabolites using chemical tests while the anti-proliferative effect against peripheral blood mononuclear were tested using the WST-1 assay. Nitric oxide production by RAW cell line exposed to extract was also tested using Griess assay as well as the production of interleukin- 2 in medium of cultured PMBC using an enzyme-linked-immunosorbent assay (ELISA) kit.

Results: Phytochemical testing of acetone leaf extracted was shown to be positive for flavonoids, saponins and tannins. The extract was shown to dose-dependently inhibited proliferation of mononuclear cells while promoting that of RAW cells (p<0.05). The production of IL-2 by mononuclear cells and nitric oxide release by RAW cells were inhibited and stimulated respectively (p<0.05) in both cell types. The extract was also shown to elicit significant anti- and pro-inflammatory potential at concentration above 20 and 40 μg/ml, in both cell types respectively.

Conclusion: Further laboratory research is required to elucidate the anti- and pro-inflammatory biochemical pathways as shown by the in vitro immune-modulatory modalities of this plant species. It is also important to further identify bioactive entities of Albizia gummifera responsible for the observed activities.

Keywords
Albizia gummifera; cell proliferation; Interleukin-2; nitric oxide2; phytochemistry

 
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