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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 12, No. 3, 2015, pp. 150-155
Bioline Code: tc15061
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 3, 2015, pp. 150-155

 en ANTIMYCOBACTERIAL, ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF ESSENTIAL OIL OF GALL OF PISTACIA ATLANTICA check for this species in other resources DESF. FROM ALGERIA
Sifi, I.; Dzoyem, J.P.; Ouinten, M.; Yousfi, M.; McGaw, L.J. & Eloff, J.N.

Abstract


Background: The aim of this study was to assess the antimycobacterial, antioxidant and the cytotoxic activities of the essential oil from the gall part of Pistacia atlantica check for this species in other resources Desf from Algeria.
Materials and Methods: The antimycobacterial activity was evaluated by the broth microdilution method against three species of mycobacteria: Mycobacterium smegmatis check for this species in other resources , Mycobacterium aurum check for this species in other resources and Mycobacterium fortuitum check for this species in other resources . Antioxidant activity was determined using free-radical scavenging assays. The safety of essential oil was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on C3A and Vero monkey kidney cells.
Results: The minimal inhibitory concentration (MIC) values ranged from 0.16 to 2.5 mg/mL with minimal bactericidal concentration (MBC) values ranged from 0.62 to >2.5 mg/mL. The antioxidant activity showed IC50 values ranged between 417.61 - >2000μg/mL and 495.6 - >2000μg/mL for DPPH and ABTS assay respectively. The cytotoxicity assay showed LC50 ranged between 26.47 to 93.64 μg/mL against Vero cells and 74.29 to 225.40 μg/mL against C3A. The results of this study show that the essential oils from the gall of P. atlantica have low toxicity and moderate activity against fast growing M. smegmatis and M. aurum.
Conclusion: These results substantiate their potential as used in the treatment of non-tuberculous mycobacterial infections. This finding is predictive of their activity against M. tuberculosis, therefore, further study might be considered to investigate the activity against pathogenic Mycobacterium strains.

Keywords
Pistacia atlantica; Essential oil; Antimycobacterial; Antioxidant; Cytotoxicity

 
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