African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 12, No. 3, 2015, pp. 173-182
Bioline Code: tc15064
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 3, 2015, pp. 173-182
© Copyright 2015 - African Journal of Traditional, Complementary and Alternative Medicines
INTERVENTION EFFECT AND DOSE-DEPENDENT RESPONSE OF TANREQING INJECTION ON AIRWAY MUCUS HYPERSECRETION IN LIPOPOLYSACCHARIDE-INDUCED RATS|
Dong, Shou-jin; Zhong, Yun-qing; Lu, Wen-ting; Yang, Kun; Liu, Mao-juan; Xiong, Xiao-ling & Mao, Bing
Background: Tanreqing injection, a Chinese herbal formulation comprising Radix Scutellariae, Fructus Forsythiae, Flos Lonicerae, Antelope
horn, and Bear bile powder, has been used to treat bronchitis and pneumonia for many years in China. However, its anti-mucus-hypersecretion
mechanism has yet not been fully interpreted. We aim to assess the effect and dose-response relationships of Tanreqing injection on
lipopolysacchaide (LPS) induced airway mucus hypersecretion in rats.
Material and methods: Forty-eight male rats were randomly divided into four groups (12 per group). A rat model of airway mucus
hypersecretion was generated with LPS. Tanreqing injection was given by intratracheal instillation, and bronchoalveolar lavage fluid (BALF)
from the right lung was collected. BALF total protein was determined by bicinchoninic acid disodium assay (BCA). Muc5ac was measured using
enzyme linked immunosorbent assay (ELISA) and immunohistochemistry. The expression of muc5ac mRNA was detected by real-time
polyermase chain reaction (RT-PCR). The middle lobe of the right lung was stained with alcian blue-periodic acid sthiff (AB-PAS) and positive
staining relative shading area examined.
Results: LPS caused airway mucus hypersecretion, The LPS-induced airway mucus hypersecretion increased beginning at 24 hr, and peaked at
96 hr. Tanreqing injection could inhibit airway mucus hypersecretion, and suppress the expression of muc5ac mRNA.
Conclusion: Tanreqing injection inhibits airway mucus hypersecretion in a certain dose-dependent trend.
Tanreqing injection; lipopolysaccharides; airway mucus hypersecretion; dose-response relationship
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