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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 12, No. 6, 2015, pp. 122-130
Bioline Code: tc15119
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 12, No. 6, 2015, pp. 122-130

 en EVALUATION OF WOUND HEALING AND ANTIBACTERIAL PROPERTIES OF METHANOLIC ROOT EXTRACT OF STEPHANIA DINKLAGEI check for this species in other resources (ENGL.) DIELS
Udegbunam, Sunday Ositadimma; Igbokwe, Nnabuike Prince; Udegbunam, Rita Ijeoma; Nnaji, Theophilus Okafor & Anyanwu, Madubuike Umunna

Abstract

Background: Stephania dinklagei check for this species in other resources Diels (Engl.) is used in folkloric medicine in Southeastern Nigeria for the treatment of wounds and some bacterial-associated infections. This study evaluated the wound healing and antibacterial potential of Stephania dinklagei to validate or invalidate its folkloric use.
Materials and Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of methanolic extract of S. dinklagei root (MESDR) against Pseudomonas aeruginosa check for this species in other resources , Bacillus subtilis check for this species in other resources , Staphylococcus aureus check for this species in other resources , Escherichia coli check for this species in other resources and Klebsiella spp. was determined by macro broth dilution. The extract at 20% and 10% were dosed orally to rats at 300mg/kg body weight (bw) in incision and dead space wound healing model to determine wound tensile strength and granulation tissue weight, respectively. The same extract concentrations were applied topically in excision wound model to determine the rate of wound contraction and epithelialization. Activities of superoxide dismutase (SOD) and catalase (CAT), and the levels of total protein (TP), malondialdehyde (MAL), hydroxyproline (HYP) and hexosamine (HEX) in excision wound biopsies were determined at days 7 and 14 post-wounding (pw). In the excision wound model, the extract concentrations were compared with gentamicin sulphate.
Results: The MIC of S. dinklagei extract against P. aeruginosa, S. aureus, B. subtilis, E. coli and Klebsiella spp. were 8mg/ml, 3 mg/ml, 5mg/ml, 6mg/ml and 7mg/ml, respectively, while the corresponding MBC were 10 mg/ml, 5 mg/ml, 7mg/ml, 8mg/ml, and 9 mg/ml, respectively. The 20% extract gave significantly (P<0.05) higher tensile strength and granulation tissue weight than the 10% and gentamicin sulphate. Wound contraction and epithelialization occurred significantly (P<0.05) better and faster in wounds of animals treated with the 20% extract and gentamicin sulphate compared to those treated with 10% extract. TP of animals treated with 20% extract and those treated with the reference drug did not vary significantly (P>0.05) at day 14 pw. SOD and CAT activities, and MDA and HEX level of all the groups did not vary significantly (P>0.05) at day 14 pw. HYP level of the extract-treated groups significantly (P<0.05) decreased against the control. No significant difference existed in HYP level between the extract-treated groups.
Conclusions: S. dinklagei possess antibacterial and wound healing properties which are comparable to those of gentamicin sulphate.

Keywords
Stephania dinklagei; wound healing; antibacterial; antioxidant

 
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