Background: E. coli
is the most common producer of extended spectrum beta lactamase enzyme (ESBL) which confers broad spectrum resistance to
antibiotics like penicillin, cephalosporin and monobactum.
Methods and Materials:
The present study was carried out at Pakistan Institute of Medical Sciences, Islamabad. The marked resistance was viewed
against amoxicilline-clavulanic acid, ceftriaxone and ceftazidime. The most effective drugs established were sulbactum-cefoparazone, amikacin,
pepircillin-tazobactum. A total of 220 samples of wide range were selected, i.e., blood, urine, pus, sputum, etc. and were analyzed using various
techniques of Gram staining and biochemical identification.
After performing antibiotic sensitivity tests, 83% samples came out to be ESBL positive and 17% were ESBL negative.
It was concluded that to ensure adequate treatment of infections arising especially from urinary pathogens and controlling spread of
bacterial resistant strains, the continuous monitoring by bacterial susceptibility testing is essential.