Background: A number of life threatening infections due to various bacterial pathogens are major cause of death in Pakistan. In a situation like
the other developing countries, there is a general increase in the drug resistance due to the irrational use of the antibiotics. The present
study was carried out to evaluate the prevalence and development of resistance in
Klebsiella pneumoniae
against commonly used
antibiotics.
Methods and Materials: A total of 200 samples from different wards of the hospitals were received by the pathology laboratory of
Pakistan Institute of Medical Sciences, Islamabad, which comprised of urine 65, blood 50 and other miscellaneous samples from
various sources.
Klebsiella pneumoniae was isolated and characterized using morphological, cultural, and biochemical methods and were further
confirmed by using commercially available API 20E kit. The isolates were subjected to culture sensitivity test using Kirby Bauer disc
diffusion method.
Results: The organism was found to be the most prevalent Gram-negative bacilli.
K. pneumoniae showed highest resistance against coamoxiclav
and cephalosporins.
Conclusion: The study revealed that ESBL producing strains were resistant to most of the beta-lactam antibiotics. This trend therefore
has become a significant problem and can be controlled with continued surveillance or monitoring for rationally improved antibiotics
usage, in order to prevent the drug resistance and re-emergence of infection.
