search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 13, No. 2, 2016, pp. 40-46
Bioline Code: tc16028
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 13, No. 2, 2016, pp. 40-46

 en PHYSIO-CHEMICAL EVALUATION AND BIOLOGICAL ACTIVITY OF AJUGA BRACTEOSA check for this species in other resources WALL AND VIOLA ODOROTO check for this species in other resources LINN
Shad, Anwar Ali; Zeeshan, M.; Fazal, Hina; Shah, Hamid Ullah; Ahmed, Shabir; Abeer, Hasem; Abd_Allah, E. F.; Ullah, Riaz; Afridi, Hamid; tariq, Akash & Asma, Muhammad Adnan

Abstract

Backgroud: Ajuga bracteosa check for this species in other resources and Viola odorata check for this species in other resources are frequently used by the native people of Swat-Pakistan for the curing of fever, malaria, cough, urinary and stomach disorders with slightly different practice of usage like raw powdered, extracts, decoction etc.
Methods and Materials: Disc Diffusion Method was used for determination of antimicrobial activities of both plants. Nutrient Agar Media was used for the culturing and growth of all microbial strains. Vitamin C and minerals contents were determined by standard method of AOAC. Na and K were analyzed by using flame photometric technique. Micro minerals i.e. “Ni, Cr, Fe, Cu, Zn, Mn, Ca, Pb, and Mg” were determined by Atomic Absorption Spectrophotometer (AAS). Total Soluble Solid (TSS) was determined by using abbe refractometer and pH was determined by using pH meter.
Results:The present study demonstrates that both plants exhibited antibacterial activities against P. aeruginos check for this species in other resources , E. coli check for this species in other resources , S. typhi, B. subtilis check for this species in other resources and S. aureus check for this species in other resources . The examined plants showed zone of inhibition for aqueous fraction (50.90, 45.90 %) against P. aeruginosa; for EtOAc fraction (41.37, 57.62%) against C. Albicans check for this species in other resources and for hexane fraction (25.86, 40.57%) against K. pneumoniae check for this species in other resources , respectively. Total of 14 different minerals (Na, K, P, Ca, Mg, Fe, Zn, Mn, Co, Cr, Ni, Cu, Pb, Cd) were determined and it was also observed that both the examined plants contained significant level of these analyzed minerals. The subject plants contained highest level of magnesium (295.75, 145.85 mg 100-1g) and calcium (212.49, 44.00 mg 100-1g) and potassium (152.6, 437.45 mg 100-1g) while moderate level P, Zn, Na and lower amount of Cd, Ni, Mn and Cu using Atomic Absorption, Flame Photometry and spectrophotometric techniques. V. odorata was found to contain a higher amount of vitamin C (64.05±12.37mg 100-1g) as compared to A. bracteosa check for this species in other resources (45.45 ± 7.29 mg 100-1 g).
Conclusion: Findings of this study can persuade researchers for future comprehensive phytochemical study of these plants using state of art techniques and instruments, which include not only isolation of secondary metabolites from these plants but biological evaluation of isolated compounds both in vivo and in vitro

Keywords
Ajuga bracteosa; Viola odorata; Antimicrobial activity; Elemental Profile

 
© Copyright 2016 - African Journal of Traditional, Complementary and Alternative Medicines
Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil