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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 13, No. 2, 2016, pp. 101-109
Bioline Code: tc16036
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 13, No. 2, 2016, pp. 101-109

 en IMPROVING THE SURVIVAL OF PROBIOTIC IN SIMULATED CONDITIONS AND AZOXYMETHANE-INDUCED COLON TUMOUR BEARING MICE USING MODIFIED CITRUS PECTIN-ALGINATE MICROENCAPSULATION
Odun-Ayo, Frederick; Mellem, John & Reddy, Lalini

Abstract

Backgroud: For a probiotic to be viable it needs to be preserved at a recommended minimum level of 6–7 log10cfu/g in the product being consumed, as suggested by the International Dairy Federation. Different biopolymer matrices have been used for encapsulation of probiotic; however, loss of viability is still a challenge.
Materials and Methods: Modified citrus pectin-alginate microbeads containing Lactobacillus acidophilus check for this species in other resources ATCC 4356 was developed. Efficiency of the microbeads was evaluated in simulated conditions of the gastrointestinal tract and in Balb/c mice induced with colon tumor. Genomic identification of faecal lactobacilli samples from treated mice was also performed.
Results: The Modified citrus pectin-alginate probiotic microbeads significantly enhanced the viability of Lactobacillus acidophilus ATCC 4356 compared to the control (p< 0.05) both in vitro and in vivo. Exposure of the modified citrus pectin-alginate microbeads to 3 hours of simulated gastric juice resulted in 82.7% survival of L. acidophilus ATCC 4356. Also, the number of faecal lactobacilli in the modified citrus pectin-alginate probiotic treated mice increased by 10.2% after 28 days.
Conclusion: Modified citrus pectin-alginate is a novel effective means of oral delivery of bacterial cells and bioactive compounds. Modified citrus pectin-alginate can be used in probiotic therapy which may improve the prevention of colon cancer.

Keywords
Modified citrus pectin; alginate; probiotic; microencapsulation

 
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