IN VITRO ANTIOXIDANT AND ANTI-ADIPOGENIC EFFECTS OF SLENDESTA, STANDARD POTATO EXTRACTS CONTAINING 5% PROTEASE INHIBITOR II|
Lee, Dae Geon; Park, Sang In; Kang, Su Jin; Kim, Joo Wan; Lee, Eun Kyoung; Song, Chang Hyun; Han, Chang Hyun; Lee, Young Joon & Ku, Sae Kwang
Backgroud: The objective of the present study is to observe the anti-adipogenic effects of Slendesta (SLD), a standard potato
protein extracts containing 5% potato protease inhibitor II (PI2) on the 3T3-L1 preadipocytes which are able to differentiate into
mature adipocytes and accumulate lipids, as an obesity model with cytotoxicity and antioxidant effects.
Materials and Methods: The cytotoxicity of SLD was observed against 3T3-L1 preadipocyte cell line by MTT assay, and also antiadipogenic
effects were observed through lipid accumulation assay during 3T3-L1 differentiation as comparing with N-Acetyl-Lcysteine
(NAC). In addition, antioxidant effects of SLD were detected by free radical scavenging capacity and superoxide dismutase
(SOD)-like activity as comparing with ascorbic acid.
Results: The SLD showed obvious cytotoxicity against 3T3-L1 pre-adipocyte cell line at higher concentrations, from 1.5 mg/ml for
72 h treatment, and the cytotoxic IC50 of SLD after 24, 48 and 72 h treatment times were detected as 10.11 ± 0.67, 5.71 ± 0.37 and
5.34 ± 0.21 mg/ml, respectively. The SLD also concentration-dependently inhibited the lipid accumulations formatted during 3T3-L1
cell differentiations. The adipogenic specific genes including PPARγ, C/EBPα, C/EBPβ and leptin were found to be reduced in SLD
and NAC-treated cells compared to control cells. Furthermore, the SLD effectively showed DPPH radical scavenging activity (IC50 =
161.98 ± 64.65 μg/ml) and SOD-like effects (IC50 = 284.54 ± 54.47 μg/ml), and the cellular ROS was significantly inhibited in the
SLD-treated cells compared to control cells.
Conclusion: The results suggest that SLD effectively inhibit the differentiations of 3T3-L1 preadipose cell probably through
antioxidant activities and direct cytotoxicity in case of higher concentration, along with satiety effects mediated by increases of
circulating cholecystokinin. These findings are considered as direct evidences that SLD may serve as a predictable functional
ingredient for obesity as an alternative therapy.
Slendesta; potato protease inhibitor II; 3T3-L1 cell; cytotoxicity; anti-adipogenic effects; antioxidant effects