Because reactive oxygen species (ROS) contribute to the pathogenesis of various acute and chronic liver diseases,
dietary antioxidants and drugs from herbal origins have been proved to be beneficial as therapeutic agents in reversing hepatotoxicity
and oxidative stress. The objective of this study was to investigate the protective effect of an aqueous extract from Smilax excelsa
Shoots and leaves against acute CCl4
-induced liver injury as well as the changes in antioxidative defense system in female wistar
Materials and Methods: S. excelsa
extract was administered orally in doses of 100, 200 and 400 mg/kg body weight, once daily for
9 days. Acute hepatic toxicity was induced by intraperitoneal injection of CCl4
(1 ml/kg) on the 10th
day. 24 h after CCl4
intoxication, biochemical and histopathological analyses were undertaken on sera and liver tissues.
challenge caused significant increases in the activities of liver enzymes as well as the levels of bilirubin,
malondialdehyde and nitric oxide, while total serum protein levels and antioxidant defense system parameters were reduced
significantly compared to the normal group. Administration of S. excelsa
extract at a dose of 400 mg/kg resulted in a suppression of
-induced lipid peroxidation and altered oxidative stress parameters to nearly normal values in comparison to CCl4
Nevertheless the extract did not reduce the extent of CCl4
-induced mild liver injury, as seen by the histopathology of liver damage.
The results of this study suggest that S. excelsa
could protect the liver tissues against CCl4
-induced oxidative stress
probably by increasing antioxidative defense activities.