African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 13, No. 4, 2016, pp. 29-33
Bioline Code: tc16084
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 13, No. 4, 2016, pp. 29-33
© Copyright 2016 - African Journal of Traditional, Complementary and Alternative Medicines
EFFECTS OF L-ASCORBIC ACID AND ALPHA-TOCOPHEROL ON BIOCHEMICAL PARAMETERS OF SWIMMING-INDUCED OXIDATIVE STRESS IN SERUM OF GUINEA PIGS|
Bursać-Mitrović, Marija; Milovanović, Dragan R.; Mitić, Radoslav; Jovanović, Danijela; Sovrlić, Miroslav; Vasiljević, Perica; Tomović, Jovica & Manojlović, Nedeljko
Background: The purpose of this study is to determine the effect of L-ascorbic acid and alpha-tocopherol as well as combination of these
vitamins with or without exposure to physical exercise on intensity of lipid peroxidation, activity of xanthine oxidase, activity of total
antioxidative system, concentration of glutathione, and activity of catalase in the serum of guinea pigs.
Materials and Methods: The experimental measurements of intensity of lipid peroxidation, activity of xanthine oxidase, activity of total
antioxidative system, concentration of glutathione, and activity of catalase were done in the serum of guinea pigs. The animals were exposed
to the test load to achieve exhaustion and the test was terminated when the animal for the third time to sink into the water.
Results: The results of this study demonstrated that endurance exercise of guinea pigs induced oxidative stress response in terms of
increased lipid peroxidation and activity of xanthine oxidase in the serum of experimental animals. Our study investigated the antioxidant
activity of L-ascorbic acid and alpha-tocopherol also measuring three protective markers in the serum: total antioxidant activity, content of
glutathione and activity of catalase. The results obtained show that the vitamins influence the concentrations of above mentioned
biochemical parameters, which points out their protective effect of swimming-induced oxidative stress.
Conclusion: Single or combined administration of L-ascorbic acid and alpha-tocopherol caused significant inhibition of these markers
indicating the important antioxidant activity of the vitamins. Results lead to conclude that the combined treatments with vitamins with or
without exposure to physical exercise showed the clear synergistic effect..
L-ascorbic acid; alpha-tocopherol; guinea pigs; oxidative stress; biochemical parameters
Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam