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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 13, No. 4, 2016, pp. 55-59
Bioline Code: tc16088
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 13, No. 4, 2016, pp. 55-59

 en ANTIMICROBIAL ACTIVITY OF GYMNOSPERMA GLUTINOSUM check for this species in other resources (SPRENG.) LESS. (ASTERACEAE) METHANOL EXTRACTS AGAINST HELICOBACTER PYLORI check for this species in other resources
Gomez-Flores, Ricardo; Espinosa-Ramos, David; Quintanilla-Licea, Ramiro; Barrón-Gonzalez, María Porfiria; Tamez-Guerra, Patricia; Tamez-Guerra, Reyes & Rodriguez-Padilla, Cristina

Abstract

Background: Prolonged use of antibiotics may lead to the selection of drug-resistant bacteria; as a result, efforts are being made to identify new and effective antimicrobial agents, particularly, from medicinal plants, against bacterial infections. Antimicrobial activity of Gymnosperma glutinosum check for this species in other resources against Helicobacter pylori check for this species in other resources has not yet been reported.
Materials and methods: The antibacterial in vitro effect of Gymnosperma glutinosum methanol leaf extracts against Helicobacter pylori (ATCC 43504) was evaluated in liquid medium by the colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay and in solid medium by the colony forming units (CFU) method.
Results: Methanol extracts significantly (p<0.05) inhibited in vitro H. pylori growth in liquid medium from 24% to 82% at concentrations ranging from 31.25 mg/ml to 500 mg/ml, respectively, and in solid medium the extracts caused significant (p<0.05) 52% and 100% bacterial growth inhibition at concentrations of 250 μg/mL and 500 μg/mL, respectively, as compared with untreated control. Methanol vehicle did not affect H. pylori growth.
Conclusion: The observed antibacterial effect of G. glutinosum extracts may be of benefit as an adjuvant treatment of diseases caused by H. pylori.

Keywords
Gymnosperma glutinosum; Helicobacter pylori; methanol extract; minimal inhibitory concentration (MIC)

 
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