African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 13, No. 4, 2016, pp. 145-156
Bioline Code: tc16098
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 13, No. 4, 2016, pp. 145-156
© Copyright 2016 - African Journal of Traditional, Complementary and Alternative Medicines
DIFFERENT CONCENTRATIONS OF SIJUNZI DECOCTION INHIBIT PROLIFERATION AND INDUCE APOPTOSIS OF HUMAN GASTRIC CANCER SGC-7901 SIDE POPULATION|
Qian, Jun; Li, Jing; Jia, Jianguang; Jin, Xin; Yu, Dajun; Guo, Chenxu; Xie, Bo & Qian, Liyu
Background: Sijunzi Decoction (SD) is a traditional Chinese medicine which is composed of Ginseng, Atractylodes, Poria and
Licorice. It is one of the commonly used Chinese traditional medicines that showed anti-gastric cancer activity in clinical studies.
Previous evidence demonstrated SD parties (Ginseng, Atractylodes, Poria, Licorice) can inhibit proliferation and induced apoptosis
for gastric cancer cell. In order to further investigate the anticancer effect of SD in gastric cancer, we observed the effects of different
concentrations of SD on proliferation and apoptosis of Side Population Cells (SP) of human gastric cancer SGC-7901.
Materials and Methods: SGC-7901 SP and Non- Side Population Cells (NSP) were sorted through flow cytometry; to detect the
changes of proliferation of SP and NSP before and after the intervention of serum containing different concentrations of SD using
cck-8 method; to detect the changes of cell cycle and apoptosis of SP and NSP before and after the intervention of serum containing
different concentrations of SD through flow cytometry; to detect the effects of serum containing different concentrations of SD on
apoptosis-related proteins Bax and Bcl-2 of SP and NSP before and after the intervention by western-blot.
Results: It was found that different concentrations of SD serum treatments inhibited cell proliferation in a time-dependent and
concentration-dependent manner. Compared with the control group (normal saline serum treatment), there were increase in G1/G0
phase population of SP and NSP, and decrease in G2/M and S phase population (P<0.05). Meanwhile, we found G1/G0 arrest
induced by different concentrations of SD serum which was followed by apoptosis in a time-dependent and concentration-dependent
manner. The apoptosis rate of SD serum treatment group was higher than the control group (P<0.05), the apoptosis rate of 48 h
treatment was higher than 24 h treatment (P<0.05), and as the SD serum concentration increases, apoptosis rate is higher and higher
(P<0.05). The expression of Bax protein of SP and NSP was higher than the control group in a time-dependent and concentration
dependent manner. The expression of Bcl-2 protein of SP and NSP was lower than the control group in a time-dependent and
concentration- dependent manner.
Conclusion: With the increase of SD serum concentrations, SD can gradually inhibits the proliferation of SP of SGC-7901 cell lines
through G1/G0 phase arrest and followed by apoptosis which involves the up-regulation of Bax and the down-regulation of Bcl-2.
Gastric Cancer; Side Population; Sijunzi Decoction; Proliferation; Apoptosis
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