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SALVIANOLIC ACID B ALLEVIATING MYOCARDIUM INJURY IN ISCHEMIA REPERFUSION RATS
Qiao, Zengyong & Xu, Yawei
Abstract
Background: Salvia miltiorrhiza (SM) Bunge is one of the widely-used Chinese medicinal herbs. Salvianolic acid B (Sal B), a bioactive
compound isolated from the Chinese herb Radix Salviae Miltiorrhizae, has been reported to exhibit anti-inflammatory and
anti-oxidantive effects.
Material and method: To study the cardioprotective effects of salvianolic acid B (Sal B) on acute myocardial ischemia reperfusion
(MIR) injury rats, on the basis of this investigation, the possible mechanism of salvianolic acid B was elucidated. Male Sprague- Dawley
rats (200–220 g) were randomly divided into five groups: sham-operated, MIR, MIR + Sal B (10 mg/kg/day, orally), MIR + Sal B (20
mg/kg/day, orally) and MIR + Sal B (30 mg/kg/day, orally). Before operation, the foregoing groups were pretreated with homologous drug
once a day for 7 days, respectively. After twelve hours in MIR, the cardioprotective effects of SPJ were evaluated by infarct size,
biochemical values, and the antioxidative and antiapoptotic relative gene expressions.
Results: Sal B significantly improved heart function and decreased infarct size; remarkably decreased levels of serum TNF-α and
IL-1β levels, increased contents of myocardium antioxidant enzymes activities; western blot results showed that Sal B ameliorate the
increased Bax and caspase-3 protins expressions and decreased Bcl-2 proteins expression and ratios of Bcl-2 to Bax.
Conclusion: In ischemic myocardium, oxidative stress caused the overgeneration and accumulation of reactive oxygen species (ROS),
which was central of cardiac ischemic injury. Sal B exerted beneficially cardioprotective effects on myocardial ischemia injury rats, mainly
scavenging oxidative stress-triggered overgeneration and accumulation of ROS, alleviating myocardial ischemia injury and cardiac cell
death.
Keywords
Immunity; Antioxidant; Rat
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