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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 13, No. 4, 2016, pp. 176-183
Bioline Code: tc16102
Full paper language: English
Document type: Research Article
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 13, No. 4, 2016, pp. 176-183

 en ANTI-INFLAMMATORYACTIVITY OF PLATYCODIN D ON ALCOHOL-INDUCED FATTY LIVER RATS VIA TLR4-MYD88-NF-κB SIGNAL PATH
Wu, Jing-tao; Yang, Gui-wen; Qi, Cui-hua; Zhou, Lei; Hu, Jian-guo & Wang, Mao-shan

Abstract

Background: The current study was designed to evaluate the effect of Platycodin D (PD), triterpenoid saponins extracted from the roots of Platycodon grandiflorum check for this species in other resources (PG) on alcohol-induced fatty liver (AFL) and investigate the possible mechanism.
Methods and Materials: A rat model was set up by feeding ethanol and fish oil to experimental rats, which then were treated with PD of 10, 20, 30 mg/kg body weight/day for 4 weeks, respectively, whereafter, liver function enzymes, endotoxin of serum and liver lipid were assayed by biochemical methods, cytokines, histochemistry of hepatic tissue, the protein expression of CD14 and TLR4, the mRNA expression of MD-2, MyD 88 and TRAF-6 were assayed.
Results: Treatment with PD on AFL rats significantly decreased the levels of serum ALT, AST and TBIL, coefficient of liver index and the hepatic tissue contents of TG, additionally and dramatically decreased serum endotoxin levels, down-regulated MD-2 and CD14 levels, as well as the mRNA expression of TLR4, MyD88 and TRAF-6, accordingly suppressed NF-κB p65 as well as endotoxin-mediated inflammatory factors such as TNF-α and IL-6.
Conclusions: Treatment with PD effectively protects against AFL through anti-inflammatory and anti-endotoxic process, and the confirmed mechanism is that PD treatment ameliorate alcoholic-induced liver injury mainly via TLR4-MyD88-NF-κB path in AFL rat.

Keywords
Platycodin D; alcoholic; fatty liver; anti-inflammation; toll-like receptor 4

 
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