THE ANTIBACTERIAL EFFECT OF URENA LOBATA L. FROMV GUANGXI ON MICE WITH STAPHYLOCOCCUS AUREUS PNEUMONIA
Yang, Yufang; Huang, Zhenguang; Zou, Xiaoqin; Zhong, Xiaobin; Liang, Xueyan & Zhou, Jinling
Background: Alcohol extract from the root of Urena lobata L. (ULL) had broad spectrum antimicrobial activity.
Studies in vitro have sho that ULL aqueous extract has antibacterial effect on S. aureusis, and the combination therapy
of the ULL aqueous extract with cefazolin sodium showed additive effect.
Materials and Methods: The mice underwent nasal inhalation with S. aureusis, a subset of mice were intra-gastric
gavage with ULL and/or intravenous injection cefazolin sodium twice daily. After being exposed to S. aureusis for 5
days, 10 days and 14 days respectively, the white blood cells count (WBC), neutrophils absolute value (NEU) and the
neutrophil percentage (NEU%) in peripheral blood, as well as the levels of serum immunoglobulin (Ig) G and IgM
were determined using commercial kits. The colony count of S. aureusis, the levels of interleukin (IL) -6 and IL-10 of
mice lung tissue were detected, and the pathological changes of lung tissue were examined using H & E staining.
Results: ULL significantly protected against S. aureusis pneumonia, as evidenced by the remarkable decrease in the
rate of S. aureusis colony count/lung weight, WBC, NEU and NEU% in peripheral blood, as well as the attenuation of
lung histopathological damage. Additionally, ULL+cefazolin could have markedly reduced the rate of S. aureusis
colony count/lung weight when compared with cefazolin. Furthermore, ULL and ULL+cefazolin both could
significantly decrease the serum levels of IgG and IgM, and the levels of IL-6, IL-10 in mice lung tissue.
Conclusion: This study first demonstrated that ULL may have potential use as a therapeutic agent for S. aureusis
pneumonia, and the roles of IgG, IgM, IL-6 and IL-10 in ULL protection against S. aureusis pneumonia remain to be
Staphylococcus aureus; pneumonia; antibacterial effect; Urena lobata L.; immunoglobulin; interleukin