Saraphi ( Mammea siamensis
) is a Thai traditional herb. In this study, the cytotoxic effects of crude ethanolic and
fractional extracts including hexane, ethyl acetate, and methanol fractions from M. siamensis
flowers were investigated in order to
determine their effect on WT1 expression in Molt4 and K562 cells and Bcr/Abl expression in K562 cells.
Materials and Methods:
The flowers of M. siamensis
were extracted using ethanol. The ethanol flower extract was further
fractionated with hexane, ethyl acetate, and methanol. Cytotoxic effects were measured by the MTT assay. Bcr/Abl and WT1
protein levels after treatments were determined by Western blotting. The total cell number was determined via
the typan blue
The hexane fraction showed the strongest cytotoxic activity on Molt4 and K562 cells, with IC50
values of 2.6 and 77.6
µg/ml, respectively. The hexane extract decreased Bcr/Abl protein expression in K562 cells by 74.6% and WT1 protein
expressions in Molt4 and K562 cells by 68.4 and 72.1%, respectively. Total cell numbers were decreased by 66.2 and 48.7% in
Molt4 and K562 cells, respectively. Mammea E/BB (main active compound) significantly decreased both Bcr/Abl and
WT1protein expressions by 75 and 49.5%, respectively when compared to vehicle control.
The hexane fraction from M. siamensis
flowers inhibited cell proliferation via
the suppression of WT1 expression in
Molt4 and K562 cells and Bcr/Abl expression in K562 cells. The active compound may be mammea E/BB. Extracts from M.
flowers show promise as naturally occurring anti-cancer drugs.