African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 14, No. 2, 2017, pp. 25-32
Bioline Code: tc17039
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 14, No. 2, 2017, pp. 25-32
© Copyright 2017 - African Journal of Traditional, Complementary and Alternative Medicines
PROTECTIVE ROLE OF CARNITINE SYNERGIZED WITH VITAMIN E AGAINST ISOPROTERENOL INDUCED CARDIAC INFARCTION IN RATS.|
Huwait, Etimad A. & Ghamdi, Maryam A. Al-
Background: The current study aimed to evaluate the role of carnitine in combination with vitamin E in protection
against myocardial infarction induced by isoproterenol (ISO) in rats.
Materials and Methods: Rats were grouped into 5 (each 10 rats): Group I. Control fed a standard diet. Group III: Rats
were injected with vitamin E (100 IU/kg bw, i.p) daily. Group IV: Rats were given carnitine (20 mg/kg bw, i.p) daily
.Group V: Rats were injected with both vitamin E (100 IU/kg bw, i.p) and carnitine (20 mg/kg bw, i.p) daily. On 7th,
8th, and 9th day, rats in groups (II-V) were injection i.p with ISO (55mg/kg b.w for successive three days). The
treatment with carnitine and vitamin E were continuous for 21 days.
Results: Canirine combined with vitamin E significantly increased coronary flow (CF) (P<0.001) in rats injected with
ISO. The recovery of rate pressure product (RPP) and left ventricular developed pressure (LVDP) were significantly
improved in treated rats in comparison to untreated. The rats administrated with ISO resulted in a significant elevation
of serum enzymes (CK-MB and LDH) compared with control group (p<0.001). However, it returned to about normal.
ISO administration resulted in a significant elevation in the levels of malondialdehyde (MDA) and nitric oxide (NO) as
compared with control (p<0.001) and a significant reduction in the activities of GSPxase and GSRase (p<0.001)
compared with control group. The levels of cardiac inflammatory markers interleukine-6 (IL-6) and tumor necrosis
factor (TNF- α) were markedly elevated in rats injected with ISO compared with control group. Vitamin E combined
with carnitine reversed these effects. However, pretreatment with vitamin E or carnitine or combined together showed a
significant reduction in MDA and NO (p<0.001) and a significant elevation in the activities of GSPxase and GSRase
(p<0.001) as compared to ISO injected group. The combined effect was more significant than individual ones.
Conclusion: Vitamin E combined with carnitine exerts potential protective effect against MI through suppression of
inflammatory mediators and enhancement of antioxidant activity.
Myocardial infarction; ISO; Vitamin E; carnitine
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