PROTECTIVE EFFECT OF VANILLIC ACID ON OVARIECTOMY-INDUCED OSTEOPOROSIS IN RATS|
Wang, Yong Gui; Jiang, Liang Bo & Gou, Bo
Background: The need for an anti-osteoporotic agent is in high demand since osteoporosis contributes to high rates of
disability or impairment (high osteoporotic fracture), morbidity and mortality. Hence, the present study is designed to
evaluate the protective effects of vanillic acid (VA) against bilateral ovariectomy-induced osteoporosis in female
Sprague-Dawley (SD) rats.
Materials and Methods: Forty healthy female adult SD rats were separated in to four groups with sham-operated
control with bilateral laprotomy (Sham; n = 10), bilateral overiectomy (OVX; n = 10) group, OVX rats were orallay
administrated with 50 mg/kg b.wt of VA (OVX + 50 VA; n = 10) or 100 mg/kg b.wt of VA (OVX + 100 VA; n = 10)
for 12 weeks (post-treatment) after 4 weeks of OVX.
Results: A significant change in the body weight gain was noted in OVX group, while treatment with VA substantially
reverted to normalcy. Meanwhile, the bone mineral density and content (BMD and BMC) were substantially improved
on supplementation with VA. Also, the bone turnover markers like calcium (Ca), phosphorous (P), osteocalcin (OC),
alkaline phosphatase (ALP) and deoxypyridinoline (DPD) and inflammatory markers (IL-1β, IL-6, and TNF-α) levels
were markedly attenuated in VA-treated rats. Moreover, the biomechanical stability was greatly ameliorated with VA
administration. Both the dose of VA showed potent anti-osteoporotic activity, but VA 100 mg showed highest
protective effects as compared with 50 mg of VA.
Conclusion: Based on the outcome, we concluded that VA 100 showed better anti-osteoporotic activity by improving
BMD and BMC as well as biomechanical stability and therefore used as an alternative therapy for treating
Osteoporosis; Vanillic acid; Ovariectomy; Antioxidant; Inflammatory markers