African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 14, No. 4, 2017, pp. 62-68
Bioline Code: tc17114
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 14, No. 4, 2017, pp. 62-68
© Copyright 2017 - African Journal of Traditional, Complementary and Alternative Medicines
IN VIVO EFFECT OF RUTA CHALEPENSIS EXTRACT ON HEPATIC CYTOCHROME 3A1 IN RATS|
Martínez-Pérez, Edith Fabiola; Hernández-Terán, Fernando & Serrano-Gallardo, Luis Benjamín
Background: Since the time when drugs began to be used, it became evident that they could produce a therapeutic effect,
but also a clinical condition of toxicity or no effect at all on humans, despite using the same doses in different patients.
Such untoward effects were termed “drug idiosyncrasy” and also “idiosyncratic drug effects”, but the factors producing
such diverse responses were never taken into account.
Materials and Methods: Ruta chalepensis L. (fringed rue) is an herbaceous plant of the Rutaceae family used in
traditional medicine due to its properties, such as its analgesic and antipyretic effects. This study used 25 male rats divided
into five groups. Plant extract was administered to Groups 1 and 2 at doses of 100 and 30 mg/kg/day, respectively, for three
days; Group 3 was administered 100 mg/kg/day of dexamethasone (DEX), as well as 100 mg/kg/day of Ruta chalepensis
extract; Group 4 was administered 100 mg/kg/day of DEX and treated as positive control; Group 5 was treated as negative
control and was administered a physiological solution. Twenty-four hours after the the last dose, the animals were
sacrificed and their livers were extracted.
Results: The aqueous extract of Ruta chalepensis, intraperitoneally administered, was able to induce cytochrome 3A1 in
doses of 30 mg/kg/day, and a greater inducing effect occurs when the plant is co-administered in doses of 100 mg/kg/day
Conclusion: This study suggests that aqueous extract of Ruta chalepensis can induce cytochrome 3a1. This study helps
provide a better understanding of CYP3a regulation. Future in vitro work is needed to determine the compounds that
produce the cytochrome modulation.
CYP3a; R. chalepensis; Wistar; qRT-PCR; Microplates; Artemia
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