African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 14, No. 4, 2017, pp. 69-79
Bioline Code: tc17115
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 14, No. 4, 2017, pp. 69-79
© Copyright 2017 - African Journal of Traditional, Complementary and Alternative Medicines
REDUCING TOXICITY AND INCREASING EFFICIENCY: ACONITINE WITH LIQUIRITIN AND GLYCYRRHETINIC ACID REGULATE CALCIUM REGULATORY PROTEINS IN RAT MYOCARDIAL CELL|
Zhang, Yuyan; Yu, Li; Jin, Weifeng; Fan, Hongjing; Li, Min; Zhou, Tianmei; Wan, Haitong & Yang, Jiehong
Background: Compatibility of Radix Aconiti Carmichaeli and Liquorice is known to treat heart diseases such as heart
failure and cardiac arrhythmias. This work answers the question that whether the active components (Aconitine,
Liquiritin and Glycyrrhetinic Acid) of Radix Aconiti Carmichaeli and Liquorice could result in regulating intracellular
calcium homeostasis and calcium cycling, and thereby verifies the therapeutic material basis.
Materials and Methods: The myocardial cells were divided into twelve groups randomly as control group, Aconitine
group, nine different dose groups that orthogonal combined with Aconitine, Liquiritin and Glycyrrhetinic Acid, and
Verapamil group. The myocardial cellular survival rate and morphology were assessed. The expression of calcium
regulation protein(RyR2、NCX1、DHPR-a1) in the myocardial cell by Western-blotting.
Results: The results exhibited that Aconitine (120 uM) significantly damaged on myocardial cell, decreased the
survival rate and expression of Na+/Ca2+ exchangers (NCX1) and dihydropteridine reducta-α1 (DHPR-a1), and
increased the expression of ryanodine receptor type2 (RyR2) obviously. The compatibility groups (Aconitine, Liquiritin
and Glycyrrhetinic Acid) all could against the damage on the myocardial cell by Aconitine at different levels.
Conclusion: Aconitine with Liquiritin and Glycyrrhetinic Acid may regulate the expression of calcium-regulated
proteins to protect myocardial cells from damage.
Aconitine; Liquiritin; Glycyrrhetinic Acid; myocardial cell; calcium regulatory
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