African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 14, No. 4, 2017, pp. 108-119
Bioline Code: tc17119
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 14, No. 4, 2017, pp. 108-119
© Copyright 2017 - African Journal of Traditional, Complementary and Alternative Medicines
PROTECTIVE EFFECT OF CAMEL MILK AS ANTI-DIABETIC SUPPLEMENT: BIOCHEMICAL, MOLECULAR AND IMMUNOHISTOCHEMICAL STUDY|
Mansour, Ahmed A.; Nassan, Mohammed A.; Saleh, Osama M. & Soliman, Mohamed M.
Background: Diabetes is a serious disease affects human health. Diabetes in advanced stages is accompanied by general
weakness and alteration in fats and carbohydrates metabolism. Recently there are some scientific trends about the usage of
camel milk (CM) in the treatment of diabetes and its associated alterations. CM contains vital active particles with insulin
like action that cure diabetes and its complications but how these effects occur, still unclear.
Materials and Methods: Seventy-five adult male rats of the albino type divided into five equal groups. Group 1 served as
a negative control (C). Group 2 was supplemented with camel milk (CM). Diabetes was induced in the remaining groups
(3, 4 and 5). Group 3 served as positive diabetic control (D). Group 4 served as diabetic and administered metformin
(D+MET). Group 5 served as diabetes and supplemented with camel milk (D+CM). Camel milk was supplemented for two
consecutive months. Serum glucose, leptin, insulin, liver, kidney, antioxidants, MDA and lipid profiles were assayed.
Tissues from liver and adipose tissues were examined using RT-PCR analysis for the changes in mRNA expression of
genes of carbohydrates and lipid metabolism. Pancreas and liver were used for immunohistochemical examination using
Results: Camel milk supplementation ameliorated serum biochemical measurements that altered after diabetes induction.
CM supplementation up-regulated mRNA expression of IRS-2, PK, and FASN genes, while down-regulated the expression
of CPT-1 to control mRNA expression level. CM did not affect the expression of PEPCK gene. On the other hand,
metformin failed to reduce the expression of CPT-1 compared to camel milk administered rats. Immunohistochemical
findings revealed that CM administration restored the immunostaining reactivity of insulin and GLUT-4 in the pancreas of
Conclusion: CM administration is of medical importance and helps physicians in the treatment of diabetes mellitus.
Camel milk; Diabetes; Gene expression; Immunohistochemistry
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