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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 14, No. 4, 2017, pp. 135-139
Bioline Code: tc17122
Full paper language: English
Document type: Study
Document available free of charge

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 14, No. 4, 2017, pp. 135-139

 en PHYTOCHEMICAL SCREENING, ANTIMICROBIAL AND CYTOTOXICITY STUDIES OF ETHANOL LEAF EXTRACT OF APHANIA SENEGALENSIS check for this species in other resources (SAPINDACEAE)
Fall, Alioune Dior; Bagla, Victor Patrick; Bassene, Emmanuel & Eloff, Jacobus Nicolaas

Abstract

Background: Aphania senegalensis (Sapindaceae) is commonly used in Senegalese traditional medicine to treat pain, inflammation, asthenia, bacterial and fungal infections.
The aim of this study was to determine the type of phytochemical constituents present in the ethanol leaf extract and its antimicrobial activity against selected bacterial and fungal pathogens.
Materials and Methods: The ethanol leaf extract of A. senegalensis was evaluated for its cytotoxic effect in the MTT assay against Vero cells. Flavonoids and tannins were the main constituents of the ethanol leaf extract.
Results: The extract inhibited the growth of the three fungal strains used in this study moderately with the lowest MIC obtained for Candida albicans check for this species in other resources (0.16 mg/mL). The extract also inhibited the growth of Aspergillus fumigatus check for this species in other resources and Cryptococcus neoformans check for this species in other resources with an MIC of 0.62 mg/mL. For bacterial pathogens, strong inhibition was obtained against Enterococcus faecalis check for this species in other resources (ATTC 29212) (MIC 0.08 mg/mL), while moderate inhibition was obtained for Escherichia coli check for this species in other resources (ATTC 25922) (MIC 0.16 mg/mL) and Staphylococcus aureus check for this species in other resources (ATTC 29213) (MIC 0.31mg/mL). The extract however did not inhibit the growth of Pseudomonas aeruginosa check for this species in other resources (ATTC 27853) at the highest concentration (2.5 mg/ml) tested. The ethanol leaf extract of A. senegalensis had a higher cytotoxicity than berberine used as the positive control (LC50 2.67±0.04 μg/mL and 9.99±0.54 μg/mL respectively). The best selectivity index values was obtained for Enterococcus faecalis (SI = 1.24), followed by Escherichia coli (SI = 0.62) for bacterial pathogens and C. albicans (SI = 0.62) for fungal pathogens.
Conclusion: The findings of this study suggest that the extracts may not be safe for use in animals infected by some pathogens.

Keywords
Aphania senegalensis; leaf; phytochemical; antimicrobial; cytotoxicity

 
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