African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
Vol. 14, No. 4, 2017, pp. 201-212
Bioline Code: tc17129
Full paper language: English
Document type: Research Article
Document available free of charge
African Journal of Traditional, Complementary and Alternative Medicines, Vol. 14, No. 4, 2017, pp. 201-212
© Copyright 2017 - African Journal of Traditional, Complementary and Alternative Medicines
ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF RHAMNAZIN ON LIPOPOLYSACCHARIDE-INDUCED ACUTE LUNG INJURY AND INFLAMMATION IN RATS|
Wu, GuoRong; Dai, XiaoPing; Li, XiangRong & Jiang, HePing
Background: Acute Lung Injury (ALI) results into severe inflammation and oxidative stress to the pulmonary tissue.
Rhamnazin is a natural flavonoid and known for its antioxidant and anti-inflammatory properties.
Materials and methods: The antioxidative and anti-inflammatory properties rhamnazin were tested for protection against
the acute lung injury. We investigated whether rhamnazin improves the lipopolysaccharide (LPS)-induced ALI in an animal
model (rat). We also studied the probable molecular mechanism of action of rhamnazin. Rhamnazin was injected
intraperitoneally (i.p.) (5, 10 and 20 mg/kg) two days before intratracheal LPS challenge (5mg/kg). The changes in lung
wet-to-dry weight ratio, LDH activity, pulmonary histopathology, BALF protein concentration, MPO activity, oxidative
stress, cytokine production were estimated.
Results: The results showed a significant attenuation of all the inflammatory parameters and a marked improvement in the
pulmonary histopathology in the animal groups pretreated with rhamnazin. The rhamnazin pretreated group also showed
activation of Nrf2 pathway and attenuation of ROS such as H2O2, MDA and hydroxyl ion. These results indicated that
rhamnazin could attenuate the symptoms of ALI in rats due to its strong antioxidant and anti-inflammatory properties.
Conclusion: The results strongly demonstrated that rhamnazin provides protection against LPS-induced ALI. The
underlying mechanisms of its anti-inflammatory action may include inhibition of Nrf2 mediated antioxidative pathway.
acute lung injury; inflammation; cytokine; BALF; flavonoid
Alternative site location: http://journals.sfu.ca/africanem/index.php/ajtcam