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VITAE Academia Biomédica Digital
Centro de Análisis de Imágenes Biomédicas Computarizadas-CAIBC0
ISSN: 1317-987x
No. 65, 2016, pp. 1-8
Bioline Code: va16003
Full paper language: Spanish
Document type: Research Article
Document available free of charge

VITAE Academia Biomédica Digital, No. 65, 2016, pp. 1-8

 en Identification of Acanthamoeba check for this species in other resources spp. isolates maintained at the amibiasis laboratory of Universidad Central de Venezuela, USING PCR-RFLP
Galindo, Mónica; Vethencourt, María Alejandra; Nessi, Anaibeth; Conde, Carlos; Luongo, Victoria; Machado, Raibeth; Ugarte, Alejandra; Galindo, María Virginia Pérez de; Wagner, Carolina & Rondón, Carmen Guzmán de

Abstract

Acanthamoeba genus includes some species related to pathology in humans. Their identification and classification was made by morphology, but it is a subjective technique and has low sensitivity and specificity. For these reasons, it is important to incorporate molecular tools. The objective of this research was to characterize morphologic and molecular features of 24 Acanthamoeba isolates, maintained at Laboratorio de Amibiasis-Escuela of Bioanálisis-UCV, previously identified as Acanthamoeba, using light microscopy and morphological criteria according to Pussard and Pons, 1977 and PCRRFLP (HinffI, HhaI and HaeIII) respectively. Morphologically, none of the strains belonged to group I. 45.8% of all isolates presented compatible morphology with Group II, other 45.8% with group III and 8.4% with both groups. Molecularly, 50% of the isolates amplified a 900 pb product and the other 50%, a 700 pb product. Isolates of Group III could not be molecularly characterized by PCRRFLP, since the digestion pattern have no coincidence with previous reports. Only 33% of strains were finally identified: A. polyphaga check for this species in other resources (A9, A12, A13, A14), A. castellanii check for this species in other resources (A26, A27, A28 and A29) and A. castellanii or A. polyphaga (A15, A25 and A30). The identified species are consistent with the most common pathogens described at the literature.

Keywords
Free living amoebae; Acanthamoeba spp; PCR-RFLP

 
 es Identificación de aislados de Acanthamoeba check for this species in other resources spp mantenidos en el laboratorio de amibiasis-UCV, mediante PCR-RFLP
Galindo, Mónica; Vethencourt, María Alejandra; Nessi, Anaibeth; Conde, Carlos; Luongo, Victoria; Machado, Raibeth; Ugarte, Alejandra; Galindo, María Virginia Pérez de; Wagner, Carolina & Rondón, Carmen Guzmán de

Resumen

Dentro del género Acanthamoeba existen especies relacionadas con patologías presentes en los humanos. Su identificación y clasificación se realizaba por morfología, pero es subjetiva y de baja sensibilidad y especificidad. Por ésto, es importante incorporar herramientas de biología molecular. El objetivo de esta investigación fue caracterizar molecular y morfológicamente 24 aislados de Acanthamoeba, mantenidos en el Laboratorio de AmibiasisEscuela de BioanálisisUCV, mediante PCRRFLP (HinffI, HhaI y HaeIII), previamente identificados morfológicamente como Acanthamoeba (Pussard y Pons 1977). Ningún aislado perteneció al grupo I. De los 24 aislados, 45,8% presentó características morfológicas compatibles con el grupo II, 45,8% con el grupo III y 8,4% con ambos grupos. Molecularmente, 50% de los aislados amplificaron productos de 900 pb y 50% de 700 pb. Los aislados del grupo III, no se pudieron caracterizar molecularmente por PCRRFLP, ya que el patrón de digestión no coincidió con patrones previamente publicados. Solo se identificó el 33% de los aislados resultando: A. polyphaga check for this species in other resources (A9, A12, A13, A14), A. castellanii check for this species in other resources (A26, A27, A28 y A29) y A. castellanii o A. polyphaga (A15, A25 y A30). Las especies identificadas coinciden con las patógenas más comúnmente descritas en la literatura.

Palabras-clave
Amibas de vida libre; Acanthamoeba spp; PCR-RFLP

 
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