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VITAE Academia Biomédica Digital
Centro de Análisis de Imágenes Biomédicas Computarizadas-CAIBC0
ISSN: 1317-987x
No. 67, 2016, pp. 1-10
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Bioline Code: va16017
Full paper language: Spanish
Document type: Research Article
Document available free of charge
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VITAE Academia Biomédica Digital, No. 67, 2016, pp. 1-10
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Biocidal effect of water-soluble extracts of propolis from Apis mellifera and Trigona sp bees over Escherichia coli and Staphylococcus aureus
Gil, Marielsa; Monsalve, Catherine; Quintana, Laura; Castrillo, Smirna; Ochoa, Greysys; Perozo, Esther & Briceño, Andris
Abstract
The main focus of this research was to evaluate the biocidal effect of the water-soluble extract of
propolis (WEP) from Apis mellifera and Trigona sp bees from San Cristobal, Tachira state,
Venezuela, on Escherichia coli and Staphylococcus aureus ATCC 25922 and 25923 respectively.
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was
determined using the tubes macrodilution method. It was observed that there was no
bacteriostatic effect but a bactericidal one on Escherichia coli MBC 50% on the WEP from Apis
mellifera, while it was obtained a MIC of 25% and a MBC of 50% on Staphylococcus aureus. As
for the WEP from Trigona sp, it was observed that there was no bacteriostatic effect for any of the
strains, but MBC of 100% (pure extract) was obtained for Escherichia coli and for
Staphylococcus aureus. It was demonstrated that the WEP from both types of bees have antimicrobial effect on the tested strains.
Keywords
Water-soluble propolis; Apis mellifera; Trigona sp; Staphylococcus aureus; Escherichia coli
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Efecto biocida de extractos hidrosolubles de propóleos de abejas Apis mellifera y Trigona sp sobre Escherichia coli y Staphylococcus aureus
Gil, Marielsa; Monsalve, Catherine; Quintana, Laura; Castrillo, Smirna; Ochoa, Greysys; Perozo, Esther & Briceño, Andris
Resumen
El objetivo de esta investigación fue evaluar el efecto biocida del extracto hidrosoluble de
propóleos (EHP) de abejas Apis mellifera y Trigonas sp. proveniente de San Cristóbal, Estado
Táchira, Venezuela sobre Escherichia coli y Staphylococcus aureus ATCC 25922 y 25923
respectivamente. Se determinó la concentración mínima inhibitoria (CMI) y la concentración
mínima bactericida (CMB) utilizando el método de macrodilución en tubos. Se observó que no
hubo efecto bacteriostático, pero si bactericida sobre Escherichia coli CMB 50% en el EHP de
Apis mellifera, mientras que en Staphylococcus aureus se obtuvo la CMI a 25% y la CMB a 50%.
En cuanto al EHP de Trigonas sp. se observó que no hubo efecto bacteriostático para ninguna
de las cepas pero se obtuvo una CMB al 100% (puro), tanto para Escherichia coli como para
Staphylococcus aureus. Se demostró que los EHP de ambos tipos de abejas tienen efecto
antimicrobiano sobre las cepas estudiadas.
Palabras-clave
Propóleos hidrosolubles; Apis mellifera; Trigonas sp; Staphylococcus aureus; Escherichia coli
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