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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 25, No. 1, 2004, pp. 69-72
Bioline Code: zr04013
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 25, No. 1, 2004, pp. 69-72

 en Screening and Cloning of RAPD Marker of Fluoride Tolerance Gene in Silkworm, Bombyx mori check for this species in other resources
XU Qing-gang CHEN Ke-ping YAO Qin LIU Xiao-yong


In this study,silkworm strain T6,tolerant to fluoride,and silkworm strain 733 xin,highly sensitive to fluoride,were used to construct the near-isogenic lines.300 random primers were used in RAPD amplification to DNAs of these lines.A molecular marker named S207 was found linked to the fluoride tolerance gene.Examination to F2 segregated individuals of the above lines verified that this molecular marker was reliable.Subsequently,the molecular marker was cloned into a T vector (pUCm-T) for sequencing.Comparing with sequences available in the GenBank showed that this molecular marker was novel.We plan to convert it into a SCAR marker to facilitate establishment of a molecular marker assisted breeding system.

Bombyx mori; Near-isogenic lines; Fluoride tolerance; RAPD marker

© Copyright 2004 Kunming Institute of Zoology, the Chinese Academy of Sciences
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