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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 25, No. 2, 2004, pp. 132-136
Bioline Code: zr04023
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 25, No. 2, 2004, pp. 132-136

 en Tissue Specific Expression of Plateau Pikas ( Ochotona curzoniae check for this species in other resources ) HIF-1α mRNA Under Normal Oxygen
ZHAO Tong-biao ZHAO Xin-quan CHANG Zhi-jie SUN Ping XU Shi-xiao ZHAO Wei

Abstract

Hypoxia inducible factor 1 (HIF-1),which is a transcription factor composed of HIF-1α and arylhydocarbon nuclear receptor translocator (ARNT),plays an important role in the signal transduction of cell response to hypoxia.Plateau pikas ( Ochotona curzoniae check for this species in other resources ),which are a kind of high anoxia-tolerance and cold adaptation species lived only above 3000 m on Qinhai-Tibet Plateau,have unusual high RMR and NST,acting mainly via increasing NST to adapt to extreme cold of the Qinghai-Tibet Plateau;and high ratio of oxygen utilizing to cope with plateau hypoxia environment.In this study,the plateau pikas were taken to Beijing and adapted under normal oxygen and room temperature for 7 days.One fragment of the plateau pika HIF-1α cDNA was obtained by RT-PCR technique and labeled with 32P as specific probe.Northern blot analysis showed that HIF-1α mRNA was expressed in many kinds of tissues and displayed a tissue specific expression:the highest in brain,next in kidney,and much less in heart,liver,and spleen.

Keywords
Plateau pika; HIF-11α; RT-PCR; Northern blotting; Qinghai-Tibet Plateau

 
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