Using RT-PCR and 3'-RACE,DMRT1
gene was partially cloned from the black porgy, Acanthopagrus schlegeli
.RT-PCR showed that DMRT1
mRNA was only expressed in the testis.When the transcript levels of DMRT1
were measured in the testis of black porgy before sex change,during sex change,or after sex change,no significant changes in the gene expression were observed.The results show that the transcript levels of DMRT1 gene in the testis of black porgy don't vary according to the changes of physiological status and structure of the testis.