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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 25, No. 2, 2004, pp. 172-176
Bioline Code: zr04031
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 25, No. 2, 2004, pp. 172-176

 en RAPD Genetic Markers in Six Species of Marine Crab
JIN Shan ZHAO Qing-song WANG Chun-lin CHEN Yin-er

Abstract

The phylogenetic relationship of six marine crabs was studied using random amplified polymorphic DNA (RAPD) technique.Predetermined optimal reaction conditions were as follows:Samples were first heated at 94°C for 4 min;and followed by 45 cycles of 1 min at 94°C,1 min at 36°C,and 2 min at 72°C then held at 72°C for 10 min.With 16 primers,123 reproducible amplified fragments ranging between 500 and 14 000 bp were acquired.In all bands,2 bands were shared by all crabs,13 shared by Charybdis japonica check for this species in other resources ,C.Acuta and C.Feriatus,16 shared by Portunus sanguinolentus check for this species in other resources and P.Trituberculatus.The amplified fragments were scored as present (1) or absent (0) for each DNA sample and an index of degree of band sharing (F) was calculated by using Nei and Li's matching coefficient method.The degree of F was 0.0606-0.8000.The value of 1-F was used to evaluate genetic distances between species.The genetic distances between species were 0.2000-0.9394.The phylogenetic trees were constructed with the method of UPGMA and NJ on the basis of genetic distances.The results from the two methods of cluster analysis are similar in general,and the relationships indicated by the phylogenetic trees show the difference between genus species.The results are in good overall agreement with classical taxonomy.

Keywords
RAPD; Marine crab; Genomic DNA; Genetic marker

 
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