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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 26, No. 3, 2005, pp. 294-299
Bioline Code: zr05011
Full paper language: Chinese
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 26, No. 3, 2005, pp. 294-299

 en Genetic Relationships Among Five Species of Mermithidae Based on RAPD
XU Fen WANG Guo-xiu ZHA Yu-ping LIU Peng CAO Wen-bo LIU Xu-sheng


In this study,the genetic relationships among five species belonging to four genera of Mermithidae were detected using RAPD. Twelve primers were screened from 47 random primers and amplified 161 bands (from 200 bp to 3 200 bp). 150 (93.17%) of these bands were polymorphic bands. The number of obtaining segments of individual primer was between 11-16,the average was 13.42. Nei's similarity coefficients and genetic distance were calculated using the RAPDistance and MEGA softwares. The phylogenetic trees were constructed with the methods of UPGMA and NJ. The results from two methods of cluster analysis were similar in general,and the relationship indicated the five species of Mermithidae could be distinguished with RAPD markers and were grouped into two distinct clusters. In the phylogenetic trees, Romanomermis culicivorax check for this species in other resources and R. wuchangensis (all parasite in larvae of mosquitoes) grouped together,then they grouped with Agamermis changshaensis check for this species in other resources (parasite in Homoptera) into one cluster. Ovomermis sinensis check for this species in other resources (parasite in Lepidopters) grouped with Amphimermis check for this species in other resources sp. (parasite in Homoptera) into one cluster. The genetic distances of five Mermithidae species showed that the relationships of intra-genus were nearer than that of inter-genus,the genetic distance between R. culicivorax and R. wuchangensis was 0.1789,but the genetic distance between inter-genus were 0.4471-0.5488. All those results suggested that RAPD was suitable for analysis of phylogenetic relationship among species of Mermithidae.

Entomogenous nematodes; Mermithidae; RAPD; Genetic relationship

© Copyright 2005 Kunming Institute of Zoology, the Chinese Academy of Sciences
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